李平, 李劲梁, 候嵘, 韩启德, 张幼怡
为观察大鼠发育成熟过程中心脏生长与其基因表达谱变化的关系，应用超声心动术检测8.10.12周龄 Wista:大鼠的心脏结构和功能指标，应用cDNA基因芯片技术观察心脏基因表达水平的变化。大鼠从8周龄生长至12周龄，体重增加约45.7 % (287士13 g二197士10 g)，前2周和后2周增加幅度相近。心脏左心室重量和室壁厚度分别增加约27. 7%(0.60士0. 03 g二0. 47士0. 02 g)和23.6%(2. 04士0.04 m m二1.65士0.13 mm)，前2周增加
Charges in the gene expression profile of the left heart verntricle duriing growth in the rat
Li Ping, LI Jin Liang, H OU Rong, HAN QI-DE, ZHANG You Yi
Institute o f Vascular Medicine，Pe ki二又University Third Hospital，The Key Laboratory of EducatiMinistry on Molecular Cardiology，Beijing 100083o
Wistar rats of 8，10 and 12- week old were chosen for study of the relationship between car diac growth and its gene expression profile changes during maturation .The ultrasonic parameters of rat hearts were recorded before sacrifice，then total RNA of left ventricle were extracted and gene expression profiles were analyzed by cDNA microarray .During growth from 8 weeks to 12 weeks，the body weight increased by 45.5(287士13 g vs 197士10 g)，and the increment in the first two- week period was equal to that of the second two- week period.The mass of left ventricle and the posterior wall thickness increased by 27. 7%(0.60士0. 03 g vs 0.47士0. 02 g) and 23. 6%(2. 04士0.04 mm vs 1 .65士0.13m m)，respectively，and their increment in the first two- week period was much more than that in the secand one .Meanwhile，the gene expression profile of the left ventricle changed significantly，which in-
volved cellular structure，metabolis m，oxidative stress，signal transduction，etc .Compared with the 8week-old rats，these genes were mostly up- regulated in 10- week old rats，while for 12- week old rats，the gene expression profile of the left ventricle recovered to the pattern of 8- week old rats again on the
whole .These results suggest that the relationship between the changes in cardiac function and gene expression profile can be analyzed comprehensively with the technique of microarray，and that the changes in gene expression profile of the left ventricle during rat maturation adapt to the physiological growth of heart，which is of benefit for keeping the metabolis m balance between materials and energy·
李平, 李劲梁, 候嵘, 韩启德, 张幼怡. 大鼠成长期左心室基因表达谱的变化[J]. 生理学报 2003; 55 (2): .
Li Ping, LI Jin Liang, H OU Rong, HAN QI-DE, ZHANG You Yi. Charges in the gene expression profile of the left heart verntricle duriing growth in the rat. Acta Physiol Sin 2003; 55 (2): (in Chinese with English abstract).