杜 平, 姚余卫, 石燕, 顾正, 王健, 孙兆贵, 左嘉客
复旦大学上海医学院，上海 20003；上海市计划生育科学研究所，国家人口和计划生育委员会计划生育药具重点实验室，上海 200032
[Uchl1 and its associated proteins were involved in spermatocyte apoptosis in mouse experimental cryptorchidism.] [Article in Chinese]
DU Ping, YAO Yu-Wei, SHI Yan, GU Zheng, WANG Jian, SUN Zhao-Gui, ZUO Jia-Ke
Shanghai Medical College, Fudan University, Shanghai 200032, China； Key Laboratory of Contraceptive Drugs and Devices of National Population and Family Planning Commission of China, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China
Uchl1 was found to be involved in spermatocyte apoptosis. The aim of the present study was to test whether Uchl1 and its associated proteins Jab1 and p27kip1 were involved in spermatogenic damages in response to heat-stress in cryptorchidism. Hematoxylin and eosin (HE) staining and DNA end labeling (TUNEL) were used to observe morphological and apoptotic characteristics of spermatogenic cells; Immunohistochemical analysis was used to detect changes of Uchl1 and its associated proteins Jab1 and p27kip1 in response to heat-stress from cryptorchidism leading to spermatocyte losses; And protein affinity analysis (pull-down) and immunofluorescence co-localization were used to verify the relevance among the three proteins in spermatocytes. The results showed that, Jab1 and p27kip1, in parallel to Uchl1, increased in spermatocytes of apoptotic appearances in response to heat-stress, but not in multinucleated giant cells; Jab1 bound to Uchl1 in testis protein extracts, and co-localized with Uchl1 and p27kip1 specifically in spermatocytes with apoptotic appearances. These results suggest that the accumulation of Uchl1 protein is involved in the heat-stress-induced spermatocyte apoptosis through a new pathway related with Jab1 and p27kip1, but not the formation of multinucleated giant cells.
通讯作者：王健,孙兆贵 E-mail: firstname.lastname@example.org, email@example.com
杜 平, 姚余卫, 石燕, 顾正, 王健, 孙兆贵, 左嘉客. Uchl1及其关联蛋白参与小鼠实验性隐睾中精母细胞凋亡[J]. 生理学报 2014; 66 (5): 528-536.
DU Ping, YAO Yu-Wei, SHI Yan, GU Zheng, WANG Jian, SUN Zhao-Gui, ZUO Jia-Ke. [Uchl1 and its associated proteins were involved in spermatocyte apoptosis in mouse experimental cryptorchidism.] [Article in Chinese]. Acta Physiol Sin 2014; 66 (5): 528-536 (in Chinese with English abstract).