茶碱改善东莨菪碱诱发的大鼠记忆障碍
金宗濂 文镜 王卫平 贺闻涛
摘 要: 用高效液相色谱测定了不同年龄SD大鼠与记忆有关脑区的腺苷和乙酰胆碱水平。结果表明, 18～20月龄鼠的脑内腺苷含量明显高于3～6月龄鼠, 而乙酰胆碱(ACh)含量却显著低于3～6月龄鼠。经腹腔给大鼠注射东莨宕碱建立近期记忆障碍模型,
同时经脑室给予茶碱后, 其跳台成绩明显优于对照组, 且脑内ACh含量亦显著升高。提示腺苷含量的随龄增加可能是老年记忆障碍的一个重要因素, 茶碱作为腺苷受体阻断剂可能通过提高脑内ACh水平起到改善东莨宕碱诱发的大鼠记忆障碍的作用,
因此茶碱可能是治疗老年记忆障碍的一种有希望的药物。
关键词: 腺苷; 茶碱; 乙酰胆碱; 东莨宕碱; 老年学习记忆障碍
学科分类号: Q427
The role of theophylline
in the improvement of scop-induced
learning and memory impairment
JIN Zong-Lian WEN Jing WANG Wei-Ping HE Wen-Tao
(College of Applied Sciences and Humanities of Beijing Union
University, Beijing 100083)
Abstract: Adenosine and ACh contents in hippocampus, striatum region
around the nucleus of basal meynert (NBM) and the frontal and temporal parts of
rat cortex were measured by a high performance liquid chromotography-electronic
detector (HPLC-ECD). Adenosine content in 18～20 month-old rats was
significantly higher than that of 3～6 month-old rats, while ACh
content in 18～20 month-old rats was lower than that of 3～6 month-old
ones. Learning and memory impairment models were established by induction of SD
rats with scopolamine (0.5 mg/kg, ip), which were injected with an adenosine
recepter antagonist theophylline (1.0 mg/5 μl, icv), and
the step-down test was performed. The error frequency in the step-down test
decreased significantly (P<0.05), and the retention time was longer than
that of control. Furthermore, ACh content increased in all the three different
brain regions. The above results suggest that adenosine may play an important
role in senile learning and memory impairment, and theophylline may improve
scopolamine-induced learning and memory impairment by increasing ACh content.
In consequence, theophylline could be a promising drug for ameliorating senile
learning and memory impairment.
Key words: adenosine; theophylline;
acetylcholine; scopolamine; senile learning and memory impairment
Supported by the Natural Science Foundation
of Beijing (No.5932007)
Corresponding author. Tel: 86-10-62004522; Fax: 86-10-62388927;
E-mail: biology@china2.ygi.edu.cn 390
金宗濂（北京联合大学应用文理学院, 北京 100083）
文镜（北京联合大学应用文理学院, 北京 100083）
王卫平（北京联合大学应用文理学院, 北京 100083）
贺闻涛（北京联合大学应用文理学院, 北京 100083）
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Received 2000-01-17 Accepted 2000-04-13