单眼视网膜摘除后鸽左右前脑基因的差异表达

许蕾　 徐磊　 李学礼　 王尧　

摘　要：利用消减抑制杂交(SSH)技术分析左眼视网膜摘除后成鸽左右前脑基因差异表达情况,对14个重组子经反Northern杂交去除假阳性,最终获得4个阳性重组子,对阳性重组子进行克隆和测序,其中PFB/SSH-8片段长226bp,它的138bp与人CaMⅠ基因外显子有85%的同源性;PFB/SSH-15片段长252bp,与nexinIalpha非表达序列有低的同源性;另外2个片段未发现有同源物。
关键词：鸽;前脑;消减抑制杂交(SSH);基因表达;反Northern杂交
分类号：Q436　文献标识码：A

Differential expression of genes in the pigeon forebrain after removal of the left retina

XU Lei（Department of Biochemistry, School of Medicine Tongji University, Shanghai 200070）　
XU Lei（Department of Biochemistry, School of Medicine Tongji University, Shanghai 200070）　
LI Xue-Li（Department of Biochemistry, School of Medicine Tongji University, Shanghai 200070）　
WANG Yao（Department of Biochemistry, School of Medicine Tongji University, Shanghai 200070）　

Abstract：Suppression subtractive hybridization (SSH) was used to analyze differential expression of genes in the pigeon forebrain after the left retina of the pigeon was removed at hatching. Four genes out of the 14 identified clones produced positive hybridization signals as shown by reverse Northern blot. Sequencing results showed that the length of PFB/SSH-8 is 226 bp, of which 138 bp produced a homology of 85% with human CaMI; the length of PFB/SSH-15 is 252 bp, showing low homology with nexin I alpha; no matched homological information was found in the remaining 2 clones.
Keywords：pigeon; forebrain; suppression subtractive hybridization (SSH); gene expression; reverse northern blot;

基金项目：This work was supported by the National Natural Science Foundation of China (No.39770244).
作者单位：许蕾（同济大学医学院生化教研室,上海 200070）　
　　　　　徐磊（同济大学医学院生化教研室,上海 200070）　
　　　　　李学礼（同济大学医学院生化教研室,上海 200070）　
　　　　　王尧（同济大学医学院生化教研室,上海 200070）　

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收稿日期：2000年7月11日

修稿日期：2000年8月12日