失血性休克引起大鼠肠系膜动脉平滑肌依钙K+通道活动改变

Hemorrhagic shock induces changes in large-conductance Ca2+-dependent K+ channel activity in mesenteric arterial smooth muscle cells of rats

开丽  胡德耀  王中峰  施玉?  刘良明 

摘 要:在由股动脉放血制备的失血性休克大鼠模型急性分离的肠系膜动脉平滑肌细胞上,利用膜片箝单通道记录技术观察了血管平滑肌依钙K+通道(BKCa)的活动。发现在对去甲肾上腺素(NE)反应性增高的休克代偿期,BKCa的开放概率(Po)和单位电导都显著较正常动物的低,Po的改变主要是由通道的慢关闭时间常数(τcs)增大引起关闭时间延长所致;而处于对NE反应性降低的休克失代偿期,BKCa的Po和单位电导都高于正常动物,Po的变化也主要是τcs减小所致。
关键词:失血性休克; 血管对去甲肾上腺素的反应性; 大电导钙激活钾通道; 血管平滑肌细胞; 肠系膜动脉
分类号:R322.74; R322.121; R364.13; R364.14; R544.2 文献标识码:A

 

基金项目:This work was supported by the National Natural Science Foundation of China (No.39870829).
作者单位:开丽(第三军医大学大坪医院野战外科研究所, 重庆 400042) 
     胡德耀(第三军医大学大坪医院野战外科研究所, 重庆 400042) 
     王中峰(中国科学院上海生命科学研究院, 上海生理研究所, 上海 200031) 
     施玉?(中国科学院上海生命科学研究院, 上海生理研究所, 上海 200031) 
     刘良明(第三军医大学大坪医院野战外科研究所, 重庆 400042) 

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收稿日期:2000年10月26日

修稿日期:2000年12月5日