鸡胚脊髓背角中神经营养活性物质的初步分离和检测

Preliminary separation and identification of the neurotrophic substances from dorsal spinal cord of chicken embryos

刘耀波  薛庆善  肖渝平  王先荣 

摘 要:9d龄鸡胚脊髓背、腹角组织提取液中,用SephadexG-75凝胶层析法分离出DⅠ和DⅡ,VⅠ和VⅡ各组蛋白质组分,体外培养检测各组分对鸡胚背根节的神经营养活性。结果表明,DⅡ组分具有明显的促背根节神经突起生长的作用,而DⅠ组分无此作用;经SDS-PAGE电泳检测,DⅡ组分中蛋白质的分子量范围是61~15kD。而腹角的分离组分VⅠ和VⅡ对背根神经节神经突起的生长并无明显作用。
关键词:脊髓背角; 神经营养活性; 背根神经节
分类号:Q954.4 文献标识码:A

 

基金项目:This work was supported by the National Nature Science Foundation of China (No.39470244).
作者单位:刘耀波(军事医学科学院基础医学研究所, 北京 100850) 
     薛庆善(中山医科大学生理学教研室, 广州 510089) 
     肖渝平(中山医科大学生物学教研室, 广州 510089) 
     王先荣(兰州医学院组织学与胚胎学教研室, 兰州 730000) 

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收稿日期:2000年10月15日

修稿日期:2000年12月15日