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颜光涛*,
郝秀华,
薛辉,
王录焕,
李英丽,
石丽萍
(解放军总医院基础医学研究所生物化学研究室，北京
100853) |
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摘 要:
为了探讨肠缺血/再灌注损伤后IL-1β基因表达和蛋白含量变化与磷脂酶A2抑制之间的关系，采用大鼠肠缺血/再灌注损伤模型,
在对照组、损伤组和磷脂酶A2抑制剂处理组动物中收集血清、肺灌洗液、腹腔灌洗液及全身重要脏器组织样品,
采用放射免疫法测定IL-1β含量,
并用RT-PCR法测定肺组织中IL-1β和Ⅱ型PLA2基因表达。结果表明,
损伤后6
h血清中IL-1β含量明显高于对照组；损伤后1和3
h, 腹腔液中IL-1β也明显高于对照组；损伤后肝组织中IL-1β水平有明显增加，而肺、肾、肠组织中IL-1β没有明显变化。损伤后肺灌洗液中IL-1β也明显高于对照组水平,
肺组织中IL-1β
mRNA表达增加,
而Ⅱ型PLA2
mRNA在损伤后表达反而有所下降。采用磷脂酶A2抑制剂氯喹、环氧化物酶抑制剂消炎痛、血小板活化因子受体阻断剂SR27417后,
IL-1β蛋白和基因表达有不同的改变。提示肠缺血/再灌注损伤后一定时间内,
肝内IL-1β
mRNA表达和血中IL-1β水平明显增高,
但是否与磷脂酶A2激活或其代谢产物的释放有关尚需进一步证明。
关键词:
肠缺血/再灌注损伤;
白介素-1β;
磷脂酶A2
学科分类号:
Q593; R392-33 |
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YAN Guang-Tao, HAO Xiu-Hua, XUE Hui, WANG Lu-Huan, LI Ying-LI, SHI Li-Ping
(Laboratory of Biochemistry, Basic Medicine Institute, General Hospital of
PLA, Beijing 100853) |
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Abstract:
The experiments were carried out to explore the interactions between IL-1
beta gene expression, protein level and phospholipase A2
（PLA2）
inhibition after intestinal ischemia/reperfusion injury. Using a rat
intestinal ischemia/reperfusion injury model, after collecting the serum,
lung lavage, abdomen cavity lavage and important organ tissue samples from
control, injury and PLA2 inhibitor treated groups, IL-1 beta level was
measured by radioimmunoassay, and the mRNA expression of IL-1beta and
type Ⅱ
PLA2 was determined by RT-PCR. After 6 hours of injury the IL-1 beta level
in serum was significantly higher than that in the control group; an
increase in IL-1 beta was also observed in abdomen cavity lavage 1 or 3
hours after injury. IL-1 beta was significantly increased in liver tissue
after injury, but was not changed obviously in the lung, kidney and
intestinal tissues. IL-1 beta in the lung lavage was significantly higher
than that of control group. The mRNA expression of IL-1beta in lung tissue
was increased after injury, but type
Ⅱ
PLA2 mRNA expression was decreased. There were different changes in IL-1
beta level and gene expression after treatments with PLA2 inhibitor
chloroquine, cyclo-oxidase inhibitor indomethacin, or PAF receptor
antagonist SR27417 respectively after injury. All these results indicate
that after intestinal ischemia/ reperfusion injury, the IL-1 beta level
and mRNA gene expression are significantly increased, however, the
relationship among IL-1 beta, PLA2 activation and its metabolites
releases remains to be further elucidated.
Key words:
intestinal ischemia/reperfusion injury; interleukin-1beta; phospholipase
A2
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