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高浓度地塞米松通过下调LMP-1的表达抑制大鼠成骨细胞的分化 |
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刘素彩1,
张志勇2,
李恩1,
(1河北医科大学生物化学教研室,
石家庄
050017;
2石家庄市第四医院,
石家庄
050011) |
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摘 要:
为探讨地塞米松(dexamethasone,
DEX)抑制成骨细胞分化的机制,
观察了不同浓度DEX对体外培养大鼠成骨细胞的碱性磷酸酶活性、
骨钙素(osteocalcin,
OC)合成、Ⅰ型胶原蛋白表达的影响，并用RT-PCR方法检测了成骨细胞中LIM矿化蛋白1
mRNA的表达量。结果显示：低浓度(10－９
mol/L)的DEX能增强碱性磷酸酶的活性、
OC的分泌和Ⅰ型胶原蛋白的表达；而高浓度(10－７
mol/L)的DEX对它们则起抑制作用，并下调成骨细胞正调节因子LMP-1
mRNA的表达。上述结果表明，低浓度的DEX促进成骨细胞的分化；高浓度的DEX则抑制成骨细胞的分化，其抑制作用可能是通过下调LMP-1
mRNA的表达而实现的。
关键词:
地塞米松;
LIM矿化蛋白1表达;
成骨细胞分化
学科分类号:
Q493.6 |
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Dexamethasone inhibits osteoblastic differentiation by down-regulation of
LIM mineralization protein 1 |
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LIU Su-Cai1, ZHANG Zhi-Yong2, LI En1,
（1Department
of Biochemistry, Hebei Medical University, Shijiazhuang 050017; 2The
Fourth Hospital of Shijiazhuang, Shijiazhuang 050011） |
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Abstract:
To investigate the
mechanisms of the inhibition of osteoblastic differentiation by
dexamethasone (DEX), the effects of different doses of DEX on the
activity of alkaline phosphatase (ALP), the synthesis of osteocalcin (OC)
and the expression of colaggen type Ⅰ were observed in the cultured rat
osteoblasts. The LIM mineralization protein-1 (LMP-1) mRNA, a positive
regulator of osteoblasts, was semi-quantified by RT-PCR. The results
showed that a lower dose (10－９ mol/L) of DEX could enhance the activity
of ALP, the synthesis of OC and the expression of collagen type Ⅰ.
However, a higher dose (10－７ mol/L) of DEX inhibited them and
down-regulated the expression of LMP-1 mRNA in osteoblasts. It is
suggested that DEX stimulates osteoblast differentiation at lower dose,
while at higher dose it inhibits osteoblast differentiation. The
inhibitory action of DEX on osteoblast differentiation might be mediated
by the down-regulation of LMP-1 mRNA.
Key words:
dexamethasone; LIM mineralization protein 1 expression;
osteoblastic differentiation
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