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DING Xue-Qin*, LIU Gui-Ming, WANG Jun-Ke, SHENG Zhuo-Ren
（Department
of Anesthesiology, The First Affiliated Hospital, China Medical
University, Shenyang 110001
）
Abstract:
The present study investigates the vasodilative action of carbon monoxide
on rat pulmonary artery in vitro. After isolation of the pulmonary artery
rings (PAR) from Wistar rats, an ACh concentration-response curve was
generated; the PARs were incubated with the NOS inhibitor L-NAME (30
μmol/L,
n=10) or the heme oxygenase inhibitor ZnPPIX (10
μmol/L)+L-NAME
(30 μmol/L,
n=10) for 30 min. After that, a second ACh concentration-response curve
was elicited. Other isolated PARs were randomly divided into two groups:
endothelium-intact group (n=8) and endothelium-denuded group (n=8). The
effect of exogenous carbon monoxide (CO) on pulmonary arterial vessel tone
was observed. The results showed that ACh induced a
concentration-dependent pulmonary vasorelaxation. This relaxation
disappeared after endothelium was denuded. The ACh induced relaxation was
attenuated after pretreatment with 30
μmol/L
L-NAME and attenuated further after pretreatment with 10
μmol/L
ZnPPIX+30 μmol/L
L-NAME. Exogenous carbon monoxide relaxed pulmonary artery in both the
endothelium-intact group and the endothelium-denuded group. These data
suggest that ZnPPIX inhibits ACh induced endothelium-dependent pulmonary
artery relaxation and that CO is an endothelium-derived relaxation factor,
and exogenous CO can relax pulmonary artery.
Key words:
carbon monoxide; nitric oxide; heme oxygenase inhibitor; vasodilation
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摘 要:
本研究观察了一氧化碳(CO)对离体大鼠肺动脉的舒张作用。制备Wistar大鼠肺动脉环,
作出ACh浓度效应曲线之后,
肺动脉环用一氧化氮合成酶抑制剂L-NAME
30 μmol/L
(n=10)或血红素氧化酶抑制剂ZnPPIX
10 μmol/L+L-NAME
30 μmol/L
(n=10) 孵育30
min, 再制备一个ACh的浓度效应曲线,
观察ZnPPIX对ACh的浓度效应曲线的影响。另取一组肺动脉环,
分为内皮完整组和去内皮组,
观察外源性CO对肺动脉环张力的影响。结果表明,
用
L-NAME孵育后,
ACh的血管舒张反应受抑,
最大抑制率为50.4±9.2%;
用ZnPPIX+L-NAME孵育后,
ACh的血管舒张反应进一步受抑,
最大抑制率为84.4±11.2%。外源性CO无论对内皮完整组还是去内皮组肺动脉都有舒张作用。本研究提示,
ZnPPIX可抑制ACh的内皮依赖性肺动脉舒张反应,
CO是一个内皮源性的血管舒张因子,
外源性CO可舒张肺动脉。
关键词:
一氧化碳;
一氧化氮;
血红素氧化酶抑制剂;
血管舒张反应
学科分类号:
Q463; R614.1 |
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