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肺内调节肽对兔支气管上皮细胞与嗜酸性粒细胞粘附功能的影响及机制探讨
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谭宇蓉,秦晓群*,管茶香,张长青
(中南大学湘雅医学院生理学教研室,
长沙
410078) |
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摘 要:
为了探讨肺内调节肽在气道各类过敏性炎症发生、发展中的作用，我们观察了血管活性肠肽(vasoactive
intestinal peptide, VIP)、表皮生长因子(epidermal
growth factor, EGF)、内皮素-1(endothelin-1,
ET-1)、降钙素基因相关肽(calcitonin
gene-related peptide, CGRP)在未受应激与臭氧应激两种条件下对支气管上皮细胞(bronchial
epithelial cell, BEC)与嗜酸性粒细胞(eosinophil,
EOS)粘附的影响。结果发现，VIP、EGF可使O3应激的BEC与EOS的粘附率下降，下调气道上皮炎症反应；ET-1、CGRP可使未受应激的BEC与EOS的粘附率增加，诱发炎症损伤反应；CGRP还能加重臭氧的应激反应；ET-1、CGRP的效应可被W7、H7阻断。抗细胞间粘附分子-1(intercellular
adhesion molecule, ICAM-1)抗体能阻断BEC与EOS的粘附，提示介导BEC与EOS粘附的粘附分子可能是ICAM-1。
关键词：肺内调节肽；支气管上皮细胞；嗜酸性粒细胞
学科分类号:
Q471; R332.2 |
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Effects of regulatory peptides on adhesion of eosinophil to bronchial
epithelial cells |
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TAN Yu-Rong, QIN Xiao-Qun*, GUAN Cha-Xiang, ZHANG Chang-Qing
(Department of Physiology, Xiang Ya Medical College, Central South
University, Changsha 410078) |
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Abstract:
To explore the roles of regulatory peptides in the process of various
anaphylactic inflammation of the airway, we observed the influence of four
peptides, i.e., vasoactive intestinal peptide (VIP), epidermal growth
factor (EGF), endothelin-1 (ET-1), and calcitonin gene-related peptide (CGRP),
on the adhesion of eosinophil (EOS) to unstimulated and O3-stressed
bronchial epithlial cells (BEC). From the experiments we observed that VIP
and EGF decreased EOS adherence to O3-stressed BEC and downregulated
airway inflammation; ET-1 and CGRP increased the adhesion of EOS to BEC in
the inflammatory process; and CGRP aggravated O3-stressed reactions.The
effects of ET-1 and CGRP were inhibited by W7 and H7. Anti-ICAM-1 antibody
inhibited the adhesion of EOS to BEC, which brings to light that EOS
adherence to BEC might be related to the expression of ICAM-1 of BEC.
Key words:
regulatory peptides; eosinophil; bronchial epithelial cells
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