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闫文生1,
阚文宏1,
黄巧冰1,
姜勇1,
王士雯2,
赵克森1,*
(1第一军医大学全军休克微循环重点实验室,
广州
510515;
2中国人民解放军总医院老年心血管病研究所,
北京
100853)
摘 要:
为研究脂多糖(lipopolysaccharide,
LPS)诱导的内毒素休克小鼠多种脏器中胞间粘附分子-1（intercellular
adhesion molecule-1, ICAM-1）表达的差异,
用5
mg/kg LPS腹腔注射小鼠后,
分别采用Western
blotting 和RT-PCR法检测组织中ICAM-1蛋白和mRNA的表达情况。
结果显示,
在正常小鼠中,
ICAM-1蛋白和mRNA的表达在肺中最多,
其次是脾,
在肾和肠有少量表达,
在肝和心中未能检出。LPS腹腔注射后6
h可诱导小鼠发生内毒素休克,
此时,
ICAM-1蛋白表达仍以在肺中最多,
在肝、脾、心、肾和肠依次减少；其中在肺、肾和脾分别比正常时增加4.5、3.0和1.5倍,
而且在正常时不能检出的肝和心中呈现阳性,
但在肠中则变化不大。脏器中ICAM-1
mRNA亦相应显著增加。上述结果表明,
在LPS诱导的内毒素休克小鼠的多种脏器中ICAM-1蛋白和mRNA表达显著增加。脏器间ICAM-1表达上调的差异可能带来内毒素休克时脏器的不同易伤性,
抑制ICAM-1的表达可能对内毒素休克的防治有重要的意义。
关键词:
内毒素休克;
脂多糖;
胞间粘附分子-1
学科分类号:
R363；R631.4
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YAN Wen-Sheng1, KAN Wen-Hong1, HUANG Qiao-Bing1, JIANG Yong1, WANG
Shi-Wen2, ZHAO Ke-Sen1,*
(1Key Laboratory of Shock and Microcirculation of PLA, The First Military
Medical University, Guangzhou 510515; 2The Institute of Geriatric
Cardiology, General Hospital of PLA, Beijing 100853)
Abstract:
To investigate and compare the expression of intercellular adhesion
molecule-1 (ICAM-1) in different organs of the mice with endotoxic shock
induced by lipopolysaccharide (LPS), protein and mRNA of ICAM-1 were
measured by Western blotting and RT-PCR respectively in different organs
of BALB/c mice administered intraperitoneally with 5 mg/kg LPS. The
results showed that the constitutive expression of ICAM-1 protein and mRNA
was the greatest in the lungs, followed by the spleen, kidney and
intestine. After LPS stimulation, the upregulation of ICAM-1 was still
greatest in the lungs, followed by the liver, spleen, heart, kidney and
intestine. Compared with the normal mice, the expression of ICAM-1 protein
in endotoxic shocked mice increased by 4.5-fold in the lungs, 3.0-fold in
the kidney, 1.5-fold in the spleen; the expression in the liver and heart
was negative under normal condition and changed into positive during
endotoxic shock; but ICAM-1 expression in the intestine did not change
significantly. The expression of ICAM-1 mRNA also increased consistently.
These data highlight that LPS can up-regulate ICAM1
protein and mRNA expression in different tissues of the mice with
endotoxic shock.The difference in ICAM1
expression among the organs may lead to different sensitivity of organ
damage in endotoxic shock. This suggests that inhibition of ICAM1
expression may be a useful principle for prevention and treatment of
endotoxic shock.
Key words:
endotoxic shock；lipopolysaccharide；intercellular
adhesion molecule1
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