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程勇1,
2,焦丽红1,刘瑞华1,王庆彬1,王红1，*,夏国良2
(1中国科学院动物研究所计划生育生殖生物学国家重点实验室，北京100080;
2中国农业大学生物学院，北京100094)
摘 要:
应用幼年小鼠经孕马血清促性腺激素（pregnant
mare's serum gonadotropin, PMSG）处理的动物模型，
研究了卵泡从发育到闭锁动态变化过程中血管紧张素Ⅱ（Ang
Ⅱ）的作用，
结果表明：(1)
24日龄小鼠给予PMSG
（10
IU/只）后6
d时,
卵巢中出现大量闭锁卵泡，颗粒细胞DNA琼脂糖电泳显示了梯形条带;
(2)随卵泡闭锁发生，
卵巢Ang
Ⅱ含量增加;
(3) Ang Ⅱ显著拮抗FSH刺激颗粒细胞雌二醇生成的作用。我们认为,
Ang
Ⅱ参与了对小鼠卵泡闭锁的调节。
关键词:
血管紧张素Ⅱ;
卵泡闭锁;
颗粒细胞;
雌二醇
学科分类号:
Q492.5; R339.22
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CHENG Yong1, 2, JIAO Li-Hong1, LIU Rui-Hua1, WANG Qing-Bin1, WANG Hong1，XIA
Guo-Liang2
(1State Key Laboratory of Reproductive Biology, Institute of Zoology,
Chinese Academy of Sciences, Beijing 100080；
2College of Biology, China Agriculture University,
Beijing 100094)
Abstract:
The effect of angiotensin
Ⅱ(Ang
Ⅱ)
on the follicular development was studied by using the animal model of
follicular atresia induced by pregnant mare's serum gonadotropin (PMSG).
The results showed that: (1) a large number of atretic follicles were
found in the ovary of 24-day-old mouse after 6-day treatment of PMSG.
Deoxyribonucleic acid (DNA) extracted from granulosa cells clearly showed
a ladder band under agarose gel electrophoresis analysis. (2) the contents
of Ang Ⅱ
in the ovary extremely increased with the development of follicular
atresia; (3) Ang
Ⅱ
significantly antagonized the stimulating effect of the
follicle-stimulating hormone (FSH) on estradiol (E2) generation of
granulosa cells. It is suggested that Ang
Ⅱ
may be involved in the regulation of follicular atresia in mouse.
Key words:
angiotensin
Ⅱ;
follicular atresia; granulosa cells; estradiol
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