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欧阳平,
彭立胜,
杨红,
吴文言,
徐安龙*
(中山大学生命科学学院,
广州
510275)
摘 要:
研究观察了重组人白介素-10
(rhIL-10)对肿瘤坏死因子(TNF-α)刺激的离体大鼠胸主动脉血管平滑肌细胞增殖、
细胞周期及对p44/p42
丝裂素活化蛋白激酶的影响。实验培养大鼠主动脉血管平滑肌细胞,
采用MTS/PES法确定血管平滑肌细胞(vascular
smooth muscle cells, VSMCs)的增殖状态;
应用流式细胞术测定细胞周期;
利用p44/42磷酸化抗MAPK抗体的蛋白免疫印迹法测定MAPK蛋白表达。结果显示:
(1) TNF-α处理组与对照组相比,
TNF-α对VSMC增殖具有明显的刺激作用(P<0.05)。rhIL-10单独应用对VSMCs生长没有影响(P>0.05)。在TNF-α刺激下,
低至10
ng/ml的rhIL-10可抑制VSMCs的生长
(P<0.05)。流式细胞术测定的结果显示,
rhIL-10分别可使TNF-α作用下的VSMC大部分处于G0/G1期,
与对照组相比有明显差异(P<0.01)。(2)
TNF-α对p44/p42
MAPK蛋白表达有显著的增强作用,
此作用可被rhIL-10抑制。结果提示,
rhIL-10可抑制TNF-α诱导的VSMC增殖及p44/p42
丝裂素活化蛋白激酶的表达。
关键词:
平滑肌,
血管;
白介素-10;
丝裂素活化蛋白激酶;
细胞增殖
学科分类号:
R541
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Recombinant human interleukin-10 inhibits vascular smooth muscle cell
proliferation induced by TNF-α
OUYANG Ping, PENG Li-Sheng, YANG Hong, WU Wen-Yan, XU An-Long*
(College of Life Science, Zhong Shan University, Guangzhou 510275)
Abstract:
Vessel injury provokes a release in proinflammatory cytokines that
influence vascular smooth muscle cell (VSMC) proliferation. The purposes
of this study was to determine the effects of recombinant human
interleukin-10 (rhIL-10) on rat vascular smooth muscle cell proliferation
and the activity of p44/p42 mitogen-activated protein kinase (MAPK)
promoted by tumor necrosis factor-α
(TNF-α).
Rat aortic VSMCs were cultured and treated with rhIL-10 with or without
TNF-α.
The proliferation of VSMCs was quantified by colormetric assay. Cell cycle
analysis was performed by flow cytometry. The p44/42 MAPK activity was
evaluated by the immunoblotting technique using anti-p44/42 phospho-MAPK
antibody. Compared to control group, TNF-α
stimulated significantly VSMC proliferation in TNF-α
group. rhIL-10 alone had no effect on VSMC gro
wth, but significantly inhibited VSMC proliferation induced by TNF-α
at a dose of 10 ng/ml. The cell number in G0/G1 phase of TNF-α
and rhIL-10 co-treatment group was higher than that of TNF-α
group (P<0.01) by flow cytometry analysis. The p44/42 MAPK activity was
significantly enhanced by TNF-α
and the TNF-α
effect was opposed by rhIL-10. It is suggested that rhIL-10 can inhibit
TNF-α
induced VSMC proliferation and phosphorylation of p44/42 MAPK.
Key words:
vascular smooth muscle; interleukin-10; mitogen-activated protein kinase;
cell proliferation
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