Received 2001-12-18 Accepted 2002-02-27

This work was partly supported by grants from the National Nature Science Foundation of China (No.39730170 and 39825109) and National Key Project of Basic Research (G1999054007).

*Corresponding author. Tel: +86-21-64041900 ext 2774; Fax: +86-21-64174597; E-mail: fysun@shmu.edu.cn

生理学报, Aug. 2002, 54 (4): 294~299

Acta Physiologica Sinica

研究论文

成年鼠缺血性脑损伤诱导nestin的表达

刘鹏翀, 陆世铎, 黄娅林, 孙凤艳*

复旦大学上海医学院医学神经生物国家重点实验室, 上海 200032

摘 要: 应用免疫组化和免疫荧光双标技术结合激光共聚焦扫描显微镜, 观察缺血性脑损伤后脑内nestin的表达及其细胞类型。实验观察结果为, 再灌后1天, 在缺血中心区可见nestin阳性突起; 再灌后3天和1周时, 除缺血中心区外, 周边Ⅰ、Ⅱ、Ⅲ区均有nestin大量表达; 而2周时仅限于周边Ⅰ区大量表达。 激光共聚焦扫描显微镜观察到, 再灌后3天, 周边Ⅰ区nestin阳性突起主要与GFAP共存; 2周时, nestin阳性突起变粗、变长, 并与NSE的共存明显增多。上述研究结果提示, 脑缺血可诱导大鼠脑缺血区域表达nestin, 该表达可能与神经细胞的修复有关。

关键词: 脑缺血; 荧光双标; 激光共聚焦扫描显微镜; 巢蛋白 (nestin)

学科分类号: Q426; R743.31

The expression of nestin in ischemia-injured brain of adult rat

LIU Peng-Chong, LU Shi-Duo, HUANG Ya-Lin, SUN Feng-Yan*

National Key Laboratory of Medical Neurobiology, Shanghai Medical College, Fudan University, Shanghai 200032

Abstract: Immunohistochemistry and double immunofluorescent labeling techniques combined with confocal laser scanning microscope analysis were used to investigate the characteristic spatial induction profile of nestin following a transient middle cerebral artery occlusion in adult rat brain. The results showed that nestin was induced in ischemic core at 1 day after reperfusion. In addition to ischemic core, the expression of nestin increased in peri-ischemic Ⅰ, Ⅱ and Ⅲ regions at 3 days and 1 week, then it decreased and narrowed along the rim of ischemic core 2 weeks after reperfusion. Double immunofluorescent labeling showed that nestin positive cells were mostly co-stained with GFAP,a astrocyte marker, in peri-ischemic Ⅰ region 3 days after reperfusion. At 2 weeks, however, nestin cells showed a long process and the cells double stained with nestin and NSE,a neuonal specific marker,increased in the ischemic brain. The results suggest that cerebral ischemia induces nestin expression in damaged neurons, which might favor the neuroprotection against ischemic damage.

Key words: cerebral ischemia; double immunofluorescent labeling; confocal laser scanning microscope; nestin Nestin