Received
2001-12-07 Accepted 2002-01-30
This work was supported by the Major
State Basic Research Development Program of People's Republic of China
(No.G2000056905) and the National Natural Science Fundation of China
(No.30070306).
*Corresponding author. Tel:
+86-21-25070262; E-mail: yuanwj@smmu.edu.cn
生理学报, Aug. 2002,
54 (4): 307~310
Acta
Physiologica Sinica
研究论文
尾加压素对新生大鼠心肌细胞一氧化氮合成的影响
李 玲1, 袁文俊1,*,
潘秀颉1, 王伟忠1, 邱景伟1, 唐朝枢2,3
1第二军医大学基础部生理学教研室, 上海 200433; 2北京大学第一医院心血管病研究所, 3北京大学医学部生理系, 北京 100038
摘 要: 应用半定量逆转录-多聚酶链反应法, 观察尾加压素(urotensinⅡ, UⅡ)对培养的新生SD大鼠心肌细胞内皮型一氧化氮合酶(endothelial nitric oxide synthase, eNOS) mRNA表达的影响, 并测定UⅡ对心肌细胞内一氧化氮合酶(nitric oxide synthase, NOS)活性和一氧化氮(nitric oxide, NO)释放的影响。结果显示: UⅡ抑制培养的新生大鼠心肌细胞eNOS mRNA表达、 抑制NOS的活性及NO释放;
0.1 µmol/L浓度的UⅡ呈时间依赖性抑制心肌细胞NOS的活性及NO生成。上述实验结果提示UⅡ的心血管作用可能与NO合成系统有关。
关键词: 尿紧张素类; 一氧化氮; 内皮型一氧化氮合酶; 心肌细胞; 逆转录-多聚酶链反应
学科分类号: Q575.04; R540.2
Effect of urotensinⅡon the nitric oxide production
in neonatal rat cardiomyocytes
LI Ling1, YUAN Wen-Jun1,*, PAN Xiu-Jie1, WANG Wei-Zhong1,
QIU Jing-Wei1,
TANG
Chao-Shu2,3
1Department of
Physiology, The Second Military
Medical University, Shanghai
200433; 2Institute of Cardiovascular Research, The First Hospital,
Peking University; 3The Department of Physiology, Peking University, Beijing 100038
Abstract: The
aim of this study was to investigate the effect of urotensinⅡ(UⅡ) on the nitric
oxide (NO) production in cultured neonatal rat cardiomyocytes. The endothelial
nitric oxide synthase (eNOS) mRNA expression was assessed by semi-quantitative
reverse transcription-polymerase chain reaction. The activity of nitric oxide synthase
(NOS) and NO content in cardiomyocytes were measured. The current results
showed that UⅡinhibited eNOS mRNA expression, the NOS activity and the NO production of cardiomyocytes. UⅡ(0.1 µmol/L) inhibited the NOS activity and the NO production
in cardiomyocytes in a time-dependent manner. These results suggest that the
cardiovascular effect of UⅡmight be partially associated with NO production in
cultured neonatal rat cardiomyocytes.
Key words:
urotensins; nitric oxide; endothelial nitric oxide synthase;
cardiomyocytes; reverse
transcription-polymerase chain reaction