Received 2001-11-30 Accepted 2002-03-05

This work was supported by the National Natural Science Foundation of China (No.39730510) and a grant from the Traditional Chinese Medicine Programs of Shanghai Medicine Bureau (Xing Lin Program) (No.2000-3).

*Corresponding author. Tel: +86-21-64041900-2521; E-mail: guojch@yahoo.com

生理学报, Aug. 2002, 54 (4): 321~324

Acta Physiologica Sinica

研究论文

电针和碱性成纤维生长因子(bFGF)对脑缺血时神经细胞的保护作用

李 荣, 郭景春*, 程介士

复旦大学上海医学院针刺原理研究所, 医学神经生物学国家重点实验室, 上海 200032

摘 要: 采用暂时性脑缺血再灌注大鼠模型, 及H & E、 TUNEL细胞染色等实验技术, 观察电针或碱性成纤维生长因子, 以及两者合用对缺血性神经细胞死亡的影响。实验结果表明, 电针与碱性成纤维生长因子合用与单纯使用电针或碱性成纤维生长因子相比,可明显减少暂时性脑缺血再灌注后神经细胞坏死和凋亡。提示碱性成纤维生长因子与电针可具有互补或加强的神经保护作用。两者合用具有一定的临床实际价值。

关键词: 脑缺血; 电针; 碱性成纤维生长因子; 神经保护作用

学科分类号: Q426; Q463

The protective effect of basic fibroblast growth factor (bFGF) and electroacupuncture on neurons against cerebral ischemia

LI Rong, GUO Jing-Chun, CHENG Jie-Shi*

National Key Laboratory of Medical Neurobiology, Institute of Acupuncture Research, Shanghai Medical College, Fudan University, Shanghai 200032

Abstract: Transient cerebral ischemia/reperfusion rat model was adopted, and the method of H&E staining, TUNEL staining (TdT-mediated dUTP Nick End Labeling) were used to observe the effect of electroacupuncture (EA) and basic fibroblast growth factor (bFGF) on neuronal death. The results evinced that the combination of EA and bFGF could evidently reduce the neuronal death, including both necrosis and apoptosis, compared with EA or bFGF application alone. It is suggested that bFGF and EA can complement each other and enhance the protective effect following cerebral ischemia.

Key words: cerebral ischemia/reperfusion; electroacupuncture (EA); basic fibroblast growth factor

(bFGF); neuro-protective effect