Received 2002-03-21 Accepted 2002-09-28

This work was supported by the National Natural Science Foundation of China (No.30271211), the Association Franco-Chinoise pourla Recherche Scientifique & Technique (PRA B97-03), and the Sicentific Research and Development Fundation of Shantou University.

*Corresponding author. Tel: +86-754-8900456; E-mail: ksli@stu.edu.cn

小鼠血浆TNF-α、NO、NOS水平与脑不对称的关系

付清玲, 李康生*

汕头大学医学院微生物学与免疫学教研室, 汕头 515031

摘要: 研究脑不对称对单核巨噬细胞(Mo/MФ)的影响。选用雌性4周龄Balb/c健康小鼠, 用伸爪取食法根据右利分将小鼠区分为左利、右利和双利三组。LPS腹腔注射两小时后取血浆检测肿瘤坏死因子-α(TNF-α)、一氧化氮(NO)、一氧化氮合酶(NOS)水平。结果显示: (1)各组间血浆TNF-α和NO、NOS水平的变化: 正常生理组NO水平为双利组高于左利组(P<0.05); 细菌脂多糖(LPS)刺激后双利组和右利组血浆TNF-α水平高于左利组(P<0.05), NO水平为双利组高于右利组(P<0.01)和左利组(P<0.05), NOS水平为在三组小鼠间无明显差别。(2) 血浆TNF-α、NO、NOS与右利分有一定的相关关系, 各项指标随右利分的变化趋势多为双利>右利和左利, 形成一以双利分段(右利分为21~29)为高峰的弓形向上的曲线, 随右利分的增加或减少曲线向两侧延伸和下降。上述结果提示, 脑不对称小鼠单核/巨噬细胞功能存在差异。脑不对称对免疫功能的影响程度与右利分有关, 各免疫指标随右利分的变化趋势多为一以双利小鼠为高峰的弓形向上的曲线。

关键词: 脑不对称; 免疫调控; 肿瘤坏死因子α; 一氧化氮; 一氧化氮合酶

学科分类号: Q426; R338.2

The correlations between the plasma TNF-α, NO, NOS levels and brain lateralization in mice

FU Qing-ling, LI Kang-sheng

Department of Microbiology and Immunology, Shantou University Medical College, Shantou, 515031

Abstract: The brain modulates the immune system in an asymmetrical way, as shown by the association between paw preference and immune response in the mice. The purpose of the present work was to study the relationship between plasma tumor necrosis factor-α (TNF-α), nitric oxide (NO), nitric oxide synthase (NOS) and brain lateralization.　In the study, paw preference test was used to select right-pawed, left-pawed and ambidextrous mice. Mice were classified as right-pawed if the right paw entry (RPE) score was equal to or greater than 30 (30~50), left-pawed if the score was equal to or less than 20 (0~20), and ambidextrous if the score was between 21 and 29. One week after the paw preference testing, the animals were injected intraperitoneally with either sterilized 0.9% saline or lipopolysaccharide (LPS) 5μg/0.5 ml NS and were killed 2 h later. Plasma was collected from each mouse. The level of plasma TNF-αwas measured with ELISA kits provided by ENDOGEN. NO and NOS levels of plasma were detected with kits from Juli Biotechnology Company. The results showed that: (1) Plasma levels of TNF-α, NO, NOS: In the normal mice, ambidextrous mice had higher NO levels compared with left-pawed mice (P<0.05). After the injection of LPS, plasma level of TNF-αwas lower in left-pawed mice compared with those of the right-pawed and ambidextrous mice; Plasma level of NO was higher in ambidextrous mice compared with those of the right- (P<0.01) and left-pawed (P<0.05), there was no significant difference in the plasma level of NOS among ambidextrous, right- and left-pawed mice. (2) Immune parameters were correlated with the RPE scores. The shape of the curve describing this relation was similar to a parabola. In general, the levels of TNF-α, NO, NOS rose along with the increase of RPE if the scores belonged to left-pawed mice. After that, they reached a top point when the scores belonged to ambidextrous mice. Then they declined along with the increase of RPE if the scores belonged to right-pawed mice. In conclusion, plasma levels of TNF-α, NO and NOS were associated with brain lateralization, suggesting that the activities of Mo/Mф were influenced by brain lateralization, and immune parameters were correlated with the RPE scores.

Key words: brain lateralization; immunomodulation; tumor necrosis factor-α; nitric oxide; nitric oxide synthase; macrophage