Received 2002-04-22 Accepted 2002-06-26

This work was supported by the National Natural Science Foundation of China (No.30140023)

*Corresponding author. Tel: +86-754-8900407; Fax: +86-754-8900405: E-mail: shoahenghe@hotmail.com

蛋白酶激活受体2(PAR-2)激动剂对肥大细胞释放类胰蛋白酶的影响

何韶衡1，*, 谢华1, 何永松2

1汕头大学医学院变态反应学与炎症学研究所, 汕头 515031; 2四川大学华西医学院检验系,

摘要: 研究反肉桂酰-亮-异亮-甘-精-亮-鸟-[酰胺] (tc-LIGRLO), 一种PAR-2激动剂对肥大细胞类胰蛋白酶释放的影响。结果显示, 经过15 min的培养, tc-LIGRLO可引起比基础分泌量增加1倍以上的类胰蛋白酶释放, 作用强度超过抗IgE抗体和钙离子导入剂 (calcium ionophore A23187, CI)。反PAR-2激动剂, 反肉桂酰-鸟-亮-精-甘-异亮-亮-[酰胺] (tc-OLRGIL)无此作用。培养时间延长到30 min时对tc-LIGRLO的作用无明显影响。其时间关系曲线表明, tc-LIGRLO的作用从1 min开始, 3 min后达高峰。结果表明, PAR-2激动剂tc-LIGRLO是一种高效类胰蛋白酶释放刺激剂, 肥大细胞上可能有PAR-2存在。

关键词: 蛋白酶激活受体2; 类胰蛋白酶; 肥大细胞

学科分类号: R33

Effect of a proteinase-activated receptor-2 (PAR-2) agonist on tryptase release from human mast cells

HE Shao-Heng1, *, XIE Hua1, HE Yong-Song2

1Allergy and Inflammation Research Institute, the Medical College of Shantou University, Shantou 515031: 2Laboratory Medicine Department of Western China Medical College, Sichuan University,

Abstract: Proteinase-activated receptor-2 (PAR-2) expression has been observed on numerous cell types. However, little is known of the functional expression of PAR-2 on human mast cells. In the current study, the actions of a PAR-2 agonist trans-cinnamoyl-Leu-Ile-Gly-Arg-Leu- Orn-amide (tc-LIGRLO) on tryptase release from dispersed human colonic mast cells were examined. The results showed that tc-LIGRLO was able to stimulate a fold increase in tryptase release over the basal level following 15 min incubation with colonic mast cells, whereas tc-OLRGIL did not have any effect on tryptase release. The potency of tc-LIGRLO appeared greater than that of anti-IgE and calcium ionophore A23187 (CI) in induction of tryptase release. Extending the incubation time to 30 min had no significant effect on the actions of tc-LIGRLO or anti-IgE. In the time course study, it was observed that tryptase release from mast cells induced by tc-LIGRLO started at 1 min and peaked at 3 min following incubation. The above-mentioned results indicate that tc-LIGRLO is a potent stimulus of tryptase release from human mast cells, which strongly suggests that PAR-2s are expressed on human mast cells.

Key words: proteinase activated receptor-2; tryptase; mast cell