生理学报Acta Physiologica Sinica, April

研究论文

肿瘤坏死因子-α对大鼠中脑神经干细胞分化和寡突胶质细胞增殖的影响

娄淑杰1,*, 顾平1,2, 徐荷3, 徐晓辉1, 王铭维2, 何成1, 路长林1

1第二军医大学神经生物学教研室, 上海 200433; 2河北医科大学第一医院神经内科, 石家庄 050013; 3第二军医大学流行病学教研室, 上海 200433

摘要: 为观察肿瘤坏死因子对神经干细胞(NSCs)分化的影响, 本研究应用体外扩增的新生大鼠中脑NSCs, 使用免疫组织化学技术, 观察了肿瘤坏死因子-α (TNF-α )对NSCs分化及其后代细胞的影响。结果显示: (1) TNF-α 可提高中脑NSCs后代中神经元和寡突胶质细胞所占的比例; (2) TNF-α 可明显诱导由NSCs分化的寡突胶质细胞增殖, 但对星形胶质细胞的增殖作用不明显。上述观察结果提示TNF-α 对NSCs的应用具有重要影响。

关键词: 肿瘤坏死因子-α; 神经干细胞; 寡突胶质细胞; 分化

中图分类号: Q426

Effect of tumor necrosis factor-α on differentiation of mesencephalic neural stem cells and proliferation of oligodendrocytes in the rat

LOU Shu-Jie1,*, GU Ping1,2, XU He3, XU Xiao-Hui1, WANG Ming-Wei2, HE Cheng1, LU Chang-Lin1

1Department of Neurobiology, Second Military Medical University, Shanghai 200433; 2Department of

Neurology, First Hospital, Medical University of Hebei, Shijiazhuang 050013; 3Department of Epidemiology, Second Military Medical University, Shanghai 200433

Abstract: To observe the influence of tumor necrosis factor-α (TNF-α) on differentiation of rat mesencephalic neural stem cells (NSCs), the numbers of neurons, astrocytes and oligodendrocytes generated from NSCs were analyzed after differentiation for 3 days by using immunocytochemistry technique. The results show that: (1) TNF-α enhanced the proportions of neurons and oligodendrocytes in progeny of NSCs; and (2) TNF-α induced the proliferation of oligodendrocytes derived from NSCs, but the proliferation of astrocytes was not influenced by TNF-α. We conclude that the TNF-α could influence the application of NSCs.

Key words: TNF-α; neural stem cells; oligodendrocytes; differentiation