生理学报Acta Physiologica Sinica, April

*Corresponding author. Tel: +86-10-62092306; Fax: +86-10-62017700; E-mail: zhangyy@bjmu.edu.cn

研究论文

大鼠成长期左心室基因表达谱的变化

李平*, 李劲梁*, 候嵘, 韩启德, 张幼怡**

北京大学第三医院血管医学研究所, 分子心血管学教育部重点实验室, 北京 100083

摘要: 为观察大鼠发育成熟过程中心脏生长与其基因表达谱变化的关系, 应用超声心动术检测8、 10、 12周龄Wistar大鼠的心脏结构和功能指标, 应用cDNA基因芯片技术观察心脏基因表达水平的变化。大鼠从8周龄生长至12周龄, 体重增加约45.7% (287±13 g vs 197±10 g), 前2周和后2周增加幅度相近。心脏左心室重量和室壁厚度分别增加约27.7% (0.60±0.03 g vs 0.47±0.02 g)和23.6% (2.04±0.04 mm vs 1.65±0.13 mm), 前2周增加幅度明显大于后2周。基因表达谱的改变涉及细胞结构、代谢、氧化应激及信号转导等多方面的基因。10周龄和8周龄大鼠比较, 变化的基因多数上调; 12周龄和10周龄大鼠比较, 基因表达谱基本又返转至8周龄水平。结果表明, 大鼠在成长期的4周内(8-12周龄), 左心室基因表达谱发生的变化适应生理性心肌生长需要。

关键词: 生理学; 基因表达; 基因芯片; 心脏; 生长

中图分类号: Q463

Changes in the gene expression profile of the left heart ventricule during growth in the rat

LI Ping*, LI Jin-Liang*, HOU Rong, HAN Qi-De, ZHANG You-Yi**

Institute of Vascular Medicine, Peking University Third Hospital, The Key Laboratory of Education Ministry on Molecular Cardiology, Beijing 100083

Abstract: Wistar rats of 8, 10 and 12-week-old were chosen for study of the relationship between cardiac growth and its gene expression profile changes during maturation. The ultrasonic parameters of rat hearts were recorded before sacrifice, then total RNA of left ventricle were extracted and gene expression profiles were analyzed by cDNA microarray. During growth from 8 weeks to 12 weeks, the body weight increased by 45.5% (287±13 g vs 197±10 g), and the increment in the first two-week period was equal to that of the second two-week period. The mass of left ventricle and the posterior wall thickness increased by 27.7% (0.60±0.03 g vs 0.47±0.02 g) and 23.6% (2.04±0.04 mm vs 1.65±0.13 mm), respectively, and their increment in the first two-week period was much more than that in the second one. Meanwhile, the gene expression profile of the left ventricle changed significantly, which involved cellular structure, metabolism, oxidative stress, signal transduction, etc. Compared with the 8-week-old rats, these genes were mostly up-regulated in 10-week-old rats, while for 12-week-old rats, the gene expression profile of the left ventricle recovered to the pattern of 8-week-old rats again on the whole. These results suggest that the relationship between the changes in cardiac function and gene expression profile can be analyzed comprehensively with the technique of microarray, and that the changes in gene expression profile of the left ventricle during rat maturation adapt to the physiological growth of heart, which is of benefit for keeping the metabolism balance between materials and energy.

Key words: physiology; gene expression; microarray; heart; growth