Acta Physiologica Sinica, April 25, 2003, 55(2): 225-231

Received 2002-08-15 Accepted 2002-10-10

Corresponding author. Tel: +86-311-6062490; Fax: +86-311-6062490; E-mail: syho@hebmu.edu.cn

Research Paper

Intrarenal artery injection of L-arginine inhibits spontaneous activity of renal afferent nerve fibers

MA Hui-Juan, LIU Yi-Xian, WU Yu-Ming, HE Rui-Rong*

Department of Physiology, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017

Abstract: The purpose of this study was to determine the effect of intrarenal artery injection of L-arginine on multi- and single-unit spontaneous discharges of renal afferent nerve fibers in anesthetized rabbits. The results obtained are as follows: (1) intrarenal artery injection of L-arginine (0.05, 0.24, and 0.48 mmol/kg) decreased the renal afferent nerve activity (ARNA) in a dose-dependent manner with arterial pressure unchanged; (2) pretreatment with a nitric oxide synthase inhibitor L-NAME (N6-nitro-L-arginine methylester, 0.11 mmol/kg), completely abolished the effect of L-arginine; and (3) intrarenal artery injection of a nitric oxide donor SIN-1 (3-morpholinosydnonimine, 3.75 μmol/kg) also resulted in an inhibition of ARNA. The results suggest that intrarenal artery injection of NO precursor (L-arginine) and donor (SIN-1) can inhibit ARNA in anesthetized rabbits.

Key words: physiology; L-arginine; unit-activity; renal afferent nerve

肾动脉内注射L-精氨酸抑制肾神经传入纤维的自发活动

马慧娟, 刘宜先, 武宇明, 何瑞荣*

河北医科大学基础医学研究所生理室, 石家庄 050017

摘要: 研究旨在应用记录肾传入神经多单位和单位放电的方法, 观察肾动脉内注射L-精氨酸对麻醉家兔肾神经传入纤维自发放电活动的影响。结果表明: (1)肾动脉内注射L-精氨酸(0.05、0.24和0.48 mmol/kg)可呈剂量依赖性地抑制肾传入纤维的活动, 而动脉血压不变; (2)静脉内预先注射一氧化氮合酶抑制剂L-NAME (0.11 mmol/kg), 可完全阻断L-精氨酸对肾传入纤维的抑制; (3)肾动脉注射一氧化氮(NO)供体SIN-1 (3.75 μmol/kg)也可抑制肾传入神经的活动。以上结果提示: 肾动脉内应用NO前体L-精氨酸和NO供体SIN-1均可抑制肾传入纤维的自发活动。

关键词: 生理学; L-精氨酸; 单位放电; 肾传入神经

中图分类号: Q463