Acta Physiologica Sinica, June 25, 2003, 55(3): 260－264
Received 2002-10-08 Accepted 2003-01-14
This work was supported by the State Major Basic Research Development Program (G2000056905) and the National Natural Science Foundation of China (30170347).
Corresponding author. Tel: +86-10-62092183; E-mail: tangchaoshu@263.net.cn
Research Paper
Levels of
adrenomedullin and proadrenomedullin N-terminal 20 peptide in myocardium and
aorta of spontaneously hypertensive rat and Wistar- Kyoto rat
QI Yong-Fen1,2, BU Ding-Fang1, SHI Yan-Rong1，LI Ju-Xiang2, PANG Yong-Zheng1,
TANG Chao-Shu1,2，*
1Institute of Cardiovascular Disease, Peking University First Hospital, Beijing 100034;
2Department of Physiology, Peking University Health Science Center, Beijing 100083
Abstract: In this study, we observed the levels of adrenomedullin (ADM) and proadrenomedullin N-terminal 20 peptide (PAMP) in myocardium and aorta of spontaneously hypertensive rats (SHRs) in comparison with Wistar-kyoto (WKY) rats. Contents of ADM and PAMP were measured by radioimmunoassay (RIA) in plasma, myocardium and aorta. The amount of Pro-ADM mRNA of myocardium and aorta was determined by competitive quantitative reverse transcription polymerase chain reaction (RT-PCR). In SHRs the amounts of Pro-ADM mRNA of myocardium and aorta were 66.7% (P<0.01) and 73% (P<0.01) higher than those in WKY rat, respectively. In SHRs, the levels of ADM in plasma, myocardium and aorta were 29%, 76.7% and 79% (all P<0.01) higher than those in WKY rats, respectively. The level of PAMP in SHRs was increased by 42.5% in plasma (P<0.01), 47.2% in myocardium (P<0.0.1) and 27.3% in aorta (P<0.05) compared to WKY rats, respectively. In addition, the ratio of ADM content to PAMP content in SHRs group was increased compared with that in WKY group (2.0±0.25 vs 1.64±0.3 and 2.2±0.18 vs 1.56±0.28，in myocardium and aorta, respectively, P<0.01).These results suggest that ProADM gene expression is up-regulated and the increase in ADM and PAMP is different in SHRs. The significance of inconsistency of increase in ADM and PAMP in SHRs needs to be further investigated.
Key words: pathophysiology; adrenomedullin; proadrenomedullin N terminal 20 peptide (PAMP); spontaneously hypertensive rat; mRNA
自发性高血压大鼠和Wistar-Kyoto大鼠心血管组织肾上腺髓质素和肾上腺随质素原N-末端20肽的水平
齐永芬1,2, 卜定方1，石彦荣1，李菊香2，庞永正1，唐朝枢1，2，*
1北京大学第一医院心血管所，北京 100034；
2北京大学医学部生理学系，北京 100083
摘要: 本工作观察了自发性高血压大鼠(SHRs)和Wistar-kyoto (WKY)大鼠心肌和主动脉肾上腺髓质素(adrenomedullin, ADM)和肾上腺髓质素原N-末端20肽(proadrenomedullin N terminal 20 peptide, PAMP)的水平。以放射免疫分析方法测定血浆、 心肌和主动脉ADM含量。用竞争性定量逆转录多聚酶链式反应(RT-PCR)方法测定心肌和主动脉ProADM mRNA含量。结果发现, SHRs心肌和主动脉ProADM mRNA水平分别比WKY大鼠高66.7% 和73%(均P<0.01)。SHRs血浆、心肌和主动脉ADM-ir含量分别较WKY大鼠高29%、76.7%和79%(均P<0.01)。SHRs血浆、心肌和主动脉PAMP-ir水平分别较WKY大鼠高42.5%(P<0.01)、47.2%(P<0.01)和27.3%(P<0.05)。另外, SHRs 的ADM 和PAMP的比值较WKY大鼠明显增高(心肌和主动脉分别为2.0±0.25 vs 1.64±0.3和 2.2±0.18 vs 1.56±0.28)。结果提示, SHRs心肌和主动脉ProADM基因表达上调, ADM和PAMP水平升高, 但二者升高的比例不一致。SHRs 的ADM和PAMP升高不一致的病理生理意义有待进一步研究。
关键词: 病理生理学; 肾上腺髓质素; 肾上腺髓质素原N末端20肽; 自发性高血压大鼠; mRNA
中图分类号: Q463