生理学报Acta Physiologica Sinica, June 25, 2003, 55(3)

研究论文

寒冷适应差异表达基因的研究

杨发青*, 钱令嘉, 王万银, 任会荣, 胥达

天津卫生学环境医学研究所, 天津 300050

摘要: 为寻找寒冷适应机体表达上调的相关基因, 以Balb/C小鼠建立寒冷适应模型, 分别提取肌肉和肝脏RNA, 运用代表性差异分析(RDA)方法, 寻找寒冷适应表达上调的相关基因片段, 进行序列分析和同源性比较。结果显示, 在肌肉和肝脏中有部分表达上调的基因片段, 经狭线杂交验证, 其中3条基因和表达存在显著差异。这些新发现的机体寒冷适应相关基因为进一步理解寒冷适应的分子机制提供了帮助。

关键词: 寒冷适应; 差异表达基因; 代表性差异分析

中图分类号: Q413

Differentially expressed genes associated with cold acclimation

YANG Fa-Qing*, QIAN Lin-Jia, WANG Wan-Ying, REN Hui-Rong, XU Da

Institute of Hygiene and Environment Medicine, Tianjin 300050

Abstract:To investigate the upregulated genes associated with cold acclimation, a cold acclimation model was established based on Balb/C mouse. mRNA of muscle and liver were isolated, and the upregulated genes of these tissues were studied by representational differential analysis (RDA). The upregulated genes then were sequenced and searched by Blast software in GenBank database. The results showed that some genes were upregulated and possibly associated with cold acclimation. Three of these genes, transferrin, fibrinogen B-beta-chains and a new gene fragment (Genbank ID:AF454762), were confirmed to be apregulated by RNA slot-blot analysis. The finding of these genes might contribute to further understanding of the molecular mechanisms of cold acclimation.

Key words: cold acclimation; differentially expressed genes; representational differential analysis