*Corresponding author. Tel: +86-351-4135052; Fax: +86-351-4083014; Email: qiaojt@public.ty.sx.cn

 

Neuroprotective effect of lysophosphatidic acid on AbP31-35-induced apoptosis in cultured cortical neurons

ZHENG Zhao-Qing1, FANG Xian-Jun2, ZHANG Yu1, QIAO Jian-Tian1,*

1Department of Neurobiology, Shanxi Medical University, Taiyuan 030001, China

2Virginia Commonwealth University, Department of Biochemistry, 1101 East Marshall Street, Richmond, VA 23298, USA.

 

ABSTRACT: It has been reported that LPA at its lower concentrations prevents apoptosis induced by serum-deprivation in cultured cortical neurons when LPA is added into the cultural medium at the same time of serum withdrawal. The present study was designed to investigate whether lysophosphatidic acid (LPA) could also block the apoptosis induced by b-amyloid peptide fragment 31~35 (AbP31~35) in cultured cortical neurons using techniques of DNA fragmentation electrophoresis, HO33342 staining, and TUNEL examinations. The results showed that pretreatment of LPA suppressed the AbP31~35-induced apoptosis only when LPA was applied to the cultured neurons with lower concentrations (1 mmol/L~10 mmol/L) and especially, with a preceding time of 12~24 h before the AbP31~35 exposure. These facts imply that LPA also acts as a neuroprotective factor against AbP31~35-induced apoptosis, though the mechanism underlying the protective action in this case may be more complex than that involved in the serum deprivation-induced apoptosis.

 

Keywords:   cultured cortical neurons; apoptosis; b-amyloid peptide fragment 31~35; lysophosphatidic acid (LPA); neuroprotective effect; mice

 

溶血磷脂酸对b-淀粉样蛋白片段AbP31~35所致离体小鼠大脑皮层神经元凋亡的神经保护作用

郑肇青1，方羡君2，张宇1，乔健天1，*

1山西医科大学神经生物学研究室，山西，太原030001；2弗吉尼亚Commonwealth大学生物化学系, Richmond, VA 23298, 美国

 

摘  要：  前已报道，溶血磷脂酸（LPA）对去血清培养所致的神经元凋亡有神经保护作用。为了进一步观察LPA是否对AbP31~35所致的神经元凋亡也起类似的作用，本研究在培养的小鼠皮层神经元应用DNA电泳分析、HO33342和TUNEL染色法等技术，对此进行了观察。结果显示，只有使用较低浓度的LPA （1 μmol/L~10 μ mol/L）、并且将此剂量的LPA比 AbP31~35提前12~24 h加入到培养液中时，才可看到LPA明显削弱了AbP31~35所致的神经元凋亡。以上结果表明，适当浓度的LPA在长时间预作用的条件下，可对AbP31~35所致的皮层神经元凋亡起保护因子或抗凋亡因子的作用，但其作用途径可能较在去血清培养所致的凋亡时更为复杂，因为在去血清的同时加入LPA就能制止去血清所致的凋亡。

 

关键词：皮层神经元；凋亡；b－淀粉样蛋白；溶血磷脂酸；神经保护作用；小鼠

中图分类号: Q421