supported by the Nation Natural Science foundation
of Shenyang (No.1022043-1-03-2).
Corresponding author. Tel: +86-24-62215682；Email：niyueqiu@163.net
中图分类号： Q437; R764.5
豚鼠庆大霉素耳中毒后HSP70 mRNA的表达
倪月秋1,*，汤浩2，符文双1
1沈阳医学院生理教研室，沈阳 110034；2中国医科大学听力研究室，沈阳 110001
摘 要： 为探讨热休克蛋白（HSP）70 mRNA在庆大霉素（GM）耳中毒中的意义，本实验选用耳廓反射灵敏的健康白色红目豚鼠（200～250 g ）20只，，雌雄不拘，随机分成两组，每组10只。实验组动物每日腹腔注射庆大霉素（GM） 100 mg/kg；对照组动物每日腹腔注射与GM等量的生理盐水2.5
ml/kg。两组动物混合饲养，均连续用药10 d。在用药前1天和停药后第1天进行听脑干反应（ABR）测试。各组豚鼠在行第二次ABR检测后，应用原位杂交（ISH）及图像分析技术观察庆大霉素耳中毒后HSP70mRNA在豚鼠耳蜗中表达。结果显示：实验组耳蜗ABR阈值明显高于对照组，差异有显著意义（P﹤0.01）；实验组豚鼠耳蜗血管纹、螺旋韧带、螺旋神经节细胞HSP70mRNA表达呈阳性，其平均灰度值较正常对照组明显减小（P﹤0.01），即GM能显著增强耳蜗HSP70mRNA的表达。本实验结果提示，庆大霉素中毒后，动物可能通过增加 HSP70mRNA在耳蜗的表达，起保护听力的作用。
关键词：耳毒性；庆大霉素；热休克蛋白；原位杂交；听脑干反应
Expression of heat
shock protein 70 mRNA in guinea pigs cochlea with ototoxicity
of gentamicin
NI Yue-Qiu1,*，TANG Hao2，FU Wen-Shuang1
1Department
of Physiology，Shenyang Medical College，Shenyang 110034；2 Hearing Research Laboratory， China Medical University，Shenyang 110001, China
Abstract: To examine
the significance of heat shock
protein 70 mRNA in ototoxicity
resulted from gentamicin（GM）, twenty healthy albino guinea pigs (200～250 g ) of either sex with a positive
Prier reflex were randomly divided into two groups. In GM group the animals
received 100 mg/kg GM daily by intraperitoneal injection for 10 d. In saline control group the animals received 2.5 ml/kg Saline daily
by intraperitoneal injection for 10 d. Auditory brainstem response (ABR) thresholds were recorded in each
animal before and 1 day after GM or saline administration. After the second ABR measurement, the
expression of HSP70 mRNA in guinea pigs cochlea was observed with in
situ hybridization and image quantitative analysis system. The results show that the threshold for ABR in the GM group was significantly higher than
that of the saline control (P﹤0.01). The expression of HSP70 mRNA was more intensive in stria vascularis,
spiral ligament and spiral ganglion cells in the GM group than that in the
control group. These results suggest
that administration of gentamicin can induce the expression of HSP
70 mRNA in guinea pigs cochlea and that this effect may protect hearing function
from ototoxicity.
Key words: ototoxicity; gentamicin; heat shock protein; in situ hybridization; auditory brainstem response