Acta Physiologica Sinica, October 25, 2005, 57 (5): 552-556

Received 2005-01-04 Accepted 2005-04-28
This work was supported by the National Natural Science Foundation of China (No. 30200094) and the National Key Project of Basic Research of China (No. 001CB510206).
^*Corresponding author. Tel: +86-10-66931304; Fax: +86-10-68213039; E-mail: Liusj@nic.bmi.ac.cn

Research Paper

An investigation on the division of neuronal PC12 cells induced by nerve growth factor

LÜ Shuang-Hong¹, YANG Yi², LIU Shao-Jun^1,*

¹Institute of Basic Medical Sciences; ² the National Center for Biomedical Analysis, Academy of Military Medical Science, Beijing 100850, China

Abstract: Neuronal PC12 cells induced by nerve growth factor (NGF) have been considered to be postmitotic and lack the ability to divide. However, in this study, we not only detected DNA synthesis but also observed cell division in some morphologically differentiated neuronal PC12 cells bearing long neurites. More interestingly, in addition to the division of perikaryon, the neurites located on the division site of the cell membrane also divided into two parts and were allocated to the two daughter cells. These results demonstrate that the morphologically differentiated neuronal PC12 cells still retain the ability to divide. This is the first report that neuronal PC12 cells as well as their neurites can divide.

Key words: PC12 cells; nerve growth factor; cell division; neurites

神经营养因子诱导分化的神经元样PC12细胞分裂的研究

吕双红¹，杨 怡²，刘少君^1,*

军事医学科学院¹基础医学研究所; ²国家生物医学分析中心，北京100850

摘 要：神经营养因子(nerve growth factor, NGF)诱导PC12细胞分化产生的神经元样细胞一直被认为属于分裂后的细胞，没有分裂能力。然而在本研究中，我们观察了一些已经发生分化的PC12细胞，这些细胞长有很长的神经突起，在形态上属于神经元样细胞。在这些细胞中，我们不仅检测到DNA合成，而且观察到这些细胞的分裂现象。更令人感兴趣的是，除了胞体发生分裂外，位于胞体分裂位置的突起也一分为二，分别分配给两个子细胞。这些结果说明，形态发生分化的神经元样 PC12细胞仍有分裂能力。本研究首次报道神经元样PC12细胞及其突起能发生分裂。

关键词：PC12细胞；神经生长因子；细胞分裂；神经突起

中图分类号：Q253