^*Corresponding author. Tel: +86-535-6672792; Fax: +86-535-6696735; E-mail: s_hushan@163.com

HB-1：an acute myeloid leukemic cell line with the capability of infiltrating into the brain in CBA/N mice

JIANG Hui-Jie¹, SUN Hu-Shan^1,*, WANG Xu-Dong ², WANG Chang-Liu¹, LIU Ze-Long¹, Hiroyuki Gonda³, Kenkichi Sugimoto³

¹College of Life Science, Ludong University, Yantai 264025, China; ²Emergency Department, Beijing Tongren Hospital, capital University of Medical Science, Beijing 100730, China; ³Department of Cell Science, Faculty of Graduated School of Science and Technology, Niigata University, Ikarashi, Niigata 950-2181, Japan

Abstract: An acute myeloid leukemic HB-1 cell line was cloned and established from the spleen cells of irradiated CBA/N mice. Acute myeloma leukemia like syndrome would be induced in normal CBA/N mice after intravenous injection of HB-1 cells, and the mice death happened within about two weeks. In general, leukemic cells transplanted into the mice would infiltrate into the hematopoietic organs, lungs, kidney and the liver. An interesting observation in our study was that HB-1 cells were present not only in the lung, kidney, and liver but also in the cerebrum and cerebellum. It was beyond our expectation that the leukemic cells could go through the blood-brain barrier in most circumstances. On the base of the observation, we expect that the HB-1 cell could be used as a very useful model to elucidate the mechanism of infiltrating the blood-brain barrier for certain type of cells.

Key words: physiology; blood-brain barrier; acute myeloid leukemia; cell line; brain; mouse

急性髓性白血病HB-1细胞系细胞具有侵入CBA/N小鼠脑部的能力

姜惠杰¹，孙虎山^1,*，王旭东²，王昌留¹，刘泽隆¹ ，Hiroyuki Gonda³ ，Kenkichi Sugimoto³

¹ 鲁东大学生命科学学院，烟台 264025；² 首都医科大学北京同仁医院，北京 100730；³ 新潟大学科学技术研究生院细胞科学系，日本新潟 950-2181

摘 要：急性髓性白血病HB-1细胞系是由辐射处理的CBA/N小鼠脾脏细胞克隆并建立起来的。静脉注射HB-1细胞到正常CBA/N小鼠体内会诱发急性髓性白血病综合症，并使小鼠2周左右死亡。一般情况下，白血病细胞被接种到小鼠后会侵入造血器官、肺、肾和肝脏。我们在研究中发现了一令人感兴趣的现象，不仅在小鼠的肺、肾和肝脏中，而且在大脑和小脑中也观察到了HB-1细胞。白血病细胞能穿过血脑屏障在正常情况下是难以理解的，因为血脑屏障可阻止血细胞进入脑内，并且严格有选择地让小分子通过。因此，HB-1细胞将是阐明形成血脑屏障的内皮细胞上的附贴分子和选择性地让特殊细胞侵入脑的一个很好的模型。

关键词：生理学；血脑屏障；急性髓性白血病；细胞系；脑；小鼠