This work was supported by (No. 2001K10-G7).
*Corresponding author. Tel: +86-29-82655181;
E-mail: songtbao@mail.xjtu.edu.cn
*通讯作者：宋天保，西安交通大学医学院人体解剖学与组织胚胎学系, 西安,
710061.电话：029-82655181 e-mail：songtbao@mail.xjtu.edu.cn
作者简介：李晖（1964-），女，安徽淮南市人，医学博士，副教授，硕士导师。研究方向：小儿认知行为与脑发育，新生儿疾病。电话：029-85056907. E-mail:huili@mail.xjtu.edu.cn
核转录因子-κB在产前应激子代大鼠海马表达的性别差异
李 晖1,2，朱忠良3，贾 宁3，白转丽3，蔡 青3，陈 蕊2，宋天保1,*，刘健康4
西安交通大学医学院1人体解剖学与组织胚胎学系；2第一附属医院儿科；3生理学与病理生理学系，西安
710061；4美国加州大学伯克利分校细胞分子生物学系，中国科学院上海生科院营养研究所，美国奥克兰儿童营养研究中心
摘 要：采用免疫组织化学和Western blot检测NF-κB p65/p50在产前应激子代海马的表达，并探讨其表达是否存在性别差异。研究结果显示，在雌性子代，中、晚期应激组海马齿状回p65表达显著低于对照组(P<0.01)，而海马各区p50表达均显著高于对照组(P<0.01)，中、晚期应激组p65和p50表达有显著差异(P<0.01)。在雄性子代，中、晚期应激组海马齿状回p65表达显著高于对照组(P<0.01)，晚期应激组海马各区p50表达均显著低于对照组(P<0.05, P<0.01)，中、晚期应激组间p65和p50表达有显著差异(P<0.01)。雌、雄子代比较，对照组雌、雄p65表达差异极显著(P<0.01)，p50仅在海马CA1区表达差异极显著(P<0.01)；晚期应激组雌、雄海马p65/p50表达均有极显著差异(P<0.01)。Western blot与免疫组织化学结果基本一致。结果表明，产前不同时期的应激显著影响子代海马NF-κB p65和p50表达，且有性别差异，这可能是产前应激对子代雌雄大鼠学习记忆能力影响差异的机制之一。
关键词：核转录因子-κB；产前应激;；性别差异；海马
中图分类号：R338
Gender-dependent
difference of NF-κB expression in the hippocampus of prenatally
stressed offspring rats
LI Hui1,2,
ZHU Zhong-Liang3, JIA Ning3, BAI Zhuan-Li3, CAI Qing3, CHEN Rui2,
SONG Tian-Bao1,*, LIU Jian-Kang4
1Department
of Human Anatomy and Histology-Embryology; 2Department of Pediatrics
of First Affiliated Hospital; 3Department of Physiology and Pathophysiology, School of Medicine, Xi’an
Jiaotong University, Xi’an
710061, China; 4Institute for Nutritional Sciences, Institutes for
Biological Sciences, Chinese Academy of Sciences; Department of Molecular and
Cell Biology, University of California, Berkeley CA; Nutritional Genomics
Center Children’s Hospital Oakland Research Institute
Abstract: In this study, immunohistochemistry
and Western blot were used to determine whether or not the expression of NF-κB in the hippocampus of prenatally
stressed offspring rats is gender-dependent. The results were as follows: In
the female offspring rats, the expressions of p65 in the hippocampal
dentate gyrus of mid-term stress (MS) and late-term
stress (LS) groups were significantly less than that in the control group (P<0.01). There was a significant
difference between MS and LS groups (P<0.01). The expressions of p50 in
all regions of hippocampus of MS and LS groups was significantly more than that
in the control group (P<0.01). A
significant difference was also present between MS and LS groups (P<0.01). In the male offspring rats, the expressions of p65 in the hippocampal
dentate gyrus in MS and LS groups were evidently more
than that in the control group (P<0.01).
There was a significant difference between MS and the LS groups (P<0.01). The expressions of p50 in
all regions of hippocampus in MS and LS groups were significantly less than
that in the control group (P<0.05,
P<0.01). There was also a
significant difference between MS and LS groups (P<0.01). In addition, in the control group the
expressions of p65 in the hippocampal dentate gyrus of female
offspring rats were significantly more than that of male ones (P<0.01). However, in LS group the expressions of p65 in the hippocampal
dentate gyrus of female offspring rats were
significantly less than that of male ones (P<0.01).
Moreover, there was no significant
difference between feamale and male offspring rats in
MS group. In the control group the gender difference in the expression
of p50 was only observed in hippocampal CA1 (P<0.01). The expressions of p50 in all regions of hippocampus of female
offspring rats were significantly more than that of male ones in LS group (P<0.01). There was no significant difference between female and male offspring rats
in MS group. The results of Western blot were similar to those of
immunohistochemical study. These results indicate
that prenatal stress in different gestational periods significantly affects the
expressions of p65 and p50 in
hippocampus, and this effect is gender-dependent. This may be one of the
mechanisms underlying the gender difference in the ability of learning and
memory of the prenatally stressed offspring rats.
Key Word: NF -κB; prenatal
stress; hippocampus; sexual distinction