This work was supported by the Doctoral Research Grant of Jinan University.

^*Corresponding author. Tel: +86-20-85225718; E-mail: tzhuwj@jnu.edu.cn

Expression of calbindin-D28k in human fallopian tubes

XU Duo¹, ZHU Wei-jie^1,*, WANG Zi-Neng²

¹Center for Reproductive Immunology Researc; ²Medical College, Jinan University, Guangzhou 510632, China

Abstract: The present study aimed at investigating the expression of calbindin-D28k (CaBP-D28k) in human fallopian tubes, which were collected from 33 childbearing age women undergoing abdominal hysterectomy with adnexectomy for benign disease in the pelvic cavity. These women had normal menstrual cycle and history of normal pregnancy. Isthmus, ampullary and umbrella segments of fallopian tubes were respectively collected. These specimens were divided into 6 groups based on their menstrual cycles: early-proliferative stage (n=6), mid-proliferative stage (n=5), late- proliferative stage (n=5), early-secretory stage (n=7), mid-secretory stage (n=5) and late-secretory stage (n=5). The expressions of CaBP-D28k protein and mRNA in fallopian tube were determined by immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR) methods. Positive expression of CaBP-D28k protein and CaBP-D28k mRNA were observed in human fallopian tube. There was no significant difference in the expression of CaBP-D28k protein among the isthmus, ampullary and umbrella segments in the same phase of menstrual cycle (P>0.05). However, in the menstrual cycle, the expression level of CaBP-D28k protein in the epithelium was the lowest during the early- and mid-proliferative stages and increased in both the late-proliferative and early-secretory stages (P<0.05), and then decreased in the mid- and late-secretory stages (P<0.05). The expressed CaBP-D28k protein was disposed to gobbets or dispersed sheets in cytoplasm in the early- and mid- proliferative stages, and showed concentrated granules on the top of cells in the late-proliferative and early-, mid-secretory stages. Then in the late-secretory stage redistribution renewed as in the early- and mid-proliferative stages. The CaBP-D28k mRNA obviously increased in the late-proliferative and early-secretory stages (P<0.05). These findings indicate that the expressions of CaBP-D28k protein and mRNA exist in human fallopian tube and exhibit a cyclic change.

Key words: fallopian tube; Calbinindin-D28k; immunohistochemistry; reverse transcription polymerase chain reaction

钙结合蛋白calbindin-D28k在人输卵管组织的表达

许 多¹，朱伟杰^1,*，王自能²

暨南大学¹生殖免疫研究中心，广州 510632；²医学院，广州 510632

摘 要：本研究旨在探讨钙结合蛋白calbindin- D28k (CaBP- D28k)蛋白及基因在育龄妇女输卵管中的表达和变化。33例月经规律、有正常生育史的育龄妇女，因子宫肌瘤、子宫腺肌症行子宫切除术(一并切除输卵管)，输卵管取材均包括峡部、壶腹部和伞部，按月经周期分为增生早期组6例，增生中期组5例、增生晚期组5例，分泌早期组7例，分泌中期组5例和分泌晚期组5例。采用免疫组织化学法和逆转录聚合酶链反应法检测CaBP-D28k在人输卵管组织中的蛋白和基因表达。结果显示，人输卵管组织中存在CaBP-D28k蛋白及mRNA表达。CaBP-D28k蛋白表达于输卵管上皮细胞的胞浆中，在月经周期同一时期，输卵管峡部、壶腹部、伞部的表达无明显差别 (P>0.05)。在月经周期中，CaBP-D28k蛋白表达在增生早、中期最低，而在增生晚期和分泌早期明显增高(P<0.05)，分泌中、晚期显著下降(P<0.05)。CaBP-D28k蛋白阳性表达分布在月经周期中出现再分布，在增生早、中期呈灶状或片状弥散分布在胞浆中，增生晚期和分泌早、中期CaBP-D28k蛋白呈聚集的颗粒状集中在细胞顶部，至分泌晚期则仍呈灶状或弥散状分布；CaBP-D28k mRNA 表达也随月经周期发生变化，在增生晚期和分泌早期明显增高(P<0.05)。结果表明，人输卵管组织中存在CaBP-F28k蛋白及mRNA表达，且具有周期性变化。

关键词：输卵管；calbindin-D28k；免疫组织化学；逆转录聚合酶链反应

中图分类号： R339.2