^*Corresponding author. NIU Wei-Zhen, Male, 59, Master Degree, Professor, Department of Physiology, Capital Medical University, Beijing 100069, Tel: 010-83911470-82, Fax: 010-83911494, Email: niuwz@126.com

First author: WEI Hua, Female, 32, Master Degree, Department of Physiology, Capital Medical University, Beijing 100069, Tel: 010-83911470-82, Fax: 010-83911494, Email: jiang-weihua@sohu.com

A ventricular pressure-clamping system for the study of mechano-electrical feedback

WEI Hua, HUANG Hai-Xia, WANG Wei, ZHANG Zhi-Fei, FU Xiao-Suo, LIU Ping, NIU Wei-Zhen^*

Department of Physiology, Capital Medical University, Beijing 100069, China

Abstract: It is necessary to control mechanical stimuli precisely in the studies of cardiac mechano-electrical feedback (MEF). In the present study a ventricular pressure-clamping system has been developed, which can be applied to isolated-perfused rabbit hearts. Controlled by a computer, this system not only can make the left ventricle follow a command defining the same pressure wave as that during a beating cycle under the physiological condition, but also deliver mechanical stimuli with a proper waveform to the ventricle at a particular time phase. This system integrates multiple functions, including perfusing, pacing, recording of electrocardiogram and monophasic action potentials, and clamping and measuring of ventricular pressures in isolated-perfused hearts. Thus, it is a distinct system for investigating the phenomena and mechanisms of cardiac MEF at the organ level.

Key words: pressure-clamp; mechano-electrical feedback; ventricle; perfusing heart

一套研究机械电反馈的心室压力钳系统

魏 华，黄海霞，王 伟，张知非，付小锁，刘 萍，钮伟真^*

首都医科大学生理学系，北京 100069

摘 要：在心脏机械电反馈的研究中准确控制机械刺激是非常重要的。本研究室构建了一套适用于离体家兔心脏的心室压力钳系统。该系统通过计算机控制压力钳，不仅能模拟正常生理条件下左心室的压力波形，还能在心室活动周期的特定时相、以适当波形对心室施加机械刺激。该系统集心脏灌流与起搏、表面心电图记录、单相动作电位记录、心室压力钳制与测定等多种功能于一体，特别适用于器官水平上观察机械电反馈现象并探讨其机制。

关键词：压力钳；机械电反馈；心室；心脏灌流