ISSN 0371-0874, CN 31-1352/Q

最新录用

XBP1在小鼠早期妊娠子宫的表达和调节

郭思维 于雯 王煦 刘健慧 张新月 何成强 丁乃峥*

山东师范大学生命科学学院,济南250014

摘要

转录因子X盒结合蛋白1(X-box binding protein-1,XBP1)在未折叠蛋白反应中起关键作用,本研究旨在分析XBP1在小鼠早期妊娠过程中的表达和调节,探讨其与胚胎着床的相关性。建立小鼠早期妊娠模型、人工诱导蜕膜化模型、发情周期模型和激素调节模型,通过免疫组织化学技术分析XBP1在各种动物模型中的表达模式,并通过RT-qPCR检测Xbp1Xbp1u Xbp1s mRNA在这些动物模型中的表达变化。结果显示,XBP1在小鼠早期妊娠子宫中呈现动态的变化,第1~4天主要表达在腔上皮和腺上皮,第5~8主要表达在蜕膜区;在人工诱导蜕膜化的子宫中XBP1主要存在于蜕膜区;在小鼠发情周期模型中,Xbp1Xbp1u Xbp1s mRNA在发情前期的表达量最高。在卵巢切除的子宫中,雌激素处理后XBP1的表达上调。以上结果表明,XBP1与小鼠胚胎着床和蜕膜化有关,其在早期妊娠子宫中表达受到雌激素调控。

关键字: 小鼠;XBP1;早期妊娠;子宫;胚胎着床;

分类号:Q25

Expression and Regulation of XBP1 in Mouse Uterus During Early Pregnancy

GUO Si-Wei YU Wen WANG Xu LIU Jian-Hui ZHANG Xin-Yue HE Cheng-Qiang DING Nai-Zheng*

College of Life Sciences, Shandong Normal University, jinan250014, China

Abstract

The transcription factor X-box binding protein-1 (XBP1) plays a key role in unfolded protein reaction. This study was aimed to investigate the expression pattern and regulation of XBP1 in the mouse uterus around mouse embryo implantation. The methods of Immunohistochemistry(IHC) and RT-qPCR were used to test XBP1 expression in mouse models undergoing early pregnancy, artificial decidualization, oestrous cycle and the ovariectomiztion. The results showed that XBP1 was spatiotemporally expressed in decidualizing stromal cells of mouse uterus during early pregnancy and artificial decidualization. During the oestrous cycle, Xbp1 mRNA was also dynamically in mouse uterus. In the ovariectomized uterus, the expression of XBP1 was up-regulated after estrogen treatment. These results suggest that XBP1 is associated with early pregnancy in mouse, and the expression of XBP1 in luminal and glandular epithelium may be regulated by estrogen.


Key words: mouse; XBP1; early pregnancy; uterus; embryo implantation;

收稿日期:2020-04-29  录用日期:2020-10-20