史瑾, 魏家绵, 沈允钢
关键词： 叶绿体; atpE基因; ε亚基; ATPase; 光合磷酸化
The properties of expressed product of chloroplast atpE gene
Shi Jin, Wei Jiamian, Shen Yungang
Shanghai Institute of Plant Physiology,The Chinese Academy of Sciences.Shanghai 200032
The maize chloroplast atpE gene, which encodes the #epsilon# subunit of CF_(1), the coupling factor, was over-expressed in E. coli NM522 and then the expressed product was purified. The purified product was found to inhibit the ATPase activity of CF_(1) activated by heat or methanol. The antiserum raised against the expressed product, stimulated ATPast activity, and also decreased the quenching of 9-aminoacridine fluorescence. This result can be explained by the antiserum specific binding of the #epsilon# subunit from the CF_(1), which alleviated the function of #epsilon# subunit. The expressed product stimulated cyclic and non-cyclic photophosphorylation and its antiserum stimulated non-cyclic photophosphorylation but inhibited cyclic photophosphorylation, which can be explained by assuming that the two types of photophosphorylation utilize differently situated CF_(1) in thylakoids. Perhaps, ATP formation by non-cyclic photophosphorylation is only synthesized by CF_(1) of unappressed part of grana thylakoids, but that by cyclic photophosphorylation is pressed part of grana thylakoids but also CF_(1) of stroma thylakoids.
Key words: Chloroplast;AtpE gene;ε subunit;ATPase;Photophosphorylation
史瑾, 魏家绵, 沈允钢. 叶绿体atpE基因表达产物的生化特性[J]. 生理学报 1996; 48 (3): .
Shi Jin, Wei Jiamian, Shen Yungang. The properties of expressed product of chloroplast atpE gene. Acta Physiol Sin 1996; 48 (3): (in Chinese with English abstract).