孙远明, 刘佩瑛, 赵玉英, 刘朝贵, 苏承刚
华南农业大学农业生物系.广东,广州 510642；西南农业大学园艺系.重庆 630716；北京医科大学药学院.北京 100083
Extraction,isolation and identification of germination inhibitors in Amorphophallus konjac corms
Sun Yuanming, Liu Peiying, Zhao Yuying, Liu Chaogui, Su Chenggang
Department of Agricultural Biology,South China Agricultural University.Guangzhou 510642,Guangdong；China；School of Pharmaceutical Sciences,Beijing Medical University.Beijing 100083
The germination inhibitors in dormant konjac corms were extracted, isolated and identified. The volatile extract from dormant konjac corms by steam distillation or refluxing with petroleum ether was found to have no effect on the germination of Chinese cabbage seeds. The other constituents in dormant konjac corms were extracted with methanol or ethanol, concentrated to aqueous phase and then separated into basic, acidic, phenolic and neutral compounds by extraction with ethyl acetate under different pH conditions. The crude acidic extract was found to inhibit the germination of Chinese cabbage and nondormant konjac seeds but the basic, phenolic and neutral extracts had no effect on the germination of the seeds of these plants. The acidic extract purified by DEAE-cellulose ion exchange column and silica gel thin-layer chromatography was found to contain abscisic acid (ABA), ferulic and oleic acids as identified by combined gas chromatography-mass spectrometry. Exogenous ABA at concentration of and above 10 mg/L and ferulic acid at 400 mg/L were found to inhibit the sprouting and growth of terminal buds of nondormant konjac corms after soaking in them for 1 h and oleic acid had no effect on it. Therefore, we suggested that ABA and ferulic acid was the inhibitors of germination in dormant konjac corms.
Key words: Amorphophallus kon jac K. Koch;Corm;Germination inhibitor;Dormancy
孙远明, 刘佩瑛, 赵玉英, 刘朝贵, 苏承刚. 花魔芋球茎发芽抑制物质的提取、分离与鉴定[J]. 生理学报 1996; 48 (3): .
Sun Yuanming, Liu Peiying, Zhao Yuying, Liu Chaogui, Su Chenggang. Extraction,isolation and identification of germination inhibitors in Amorphophallus konjac corms. Acta Physiol Sin 1996; 48 (3): (in Chinese with English abstract).