ISSN 0371-0874, CN 31-1352/Q



储祥平 李鹏 徐宁善

上海医科大学生理学教研室. 上海 200032



关键词: 乙酰胆碱; 延髓脑片; 延髓头端腹外侧区; 自发单位放电; M受体亚型

Effects of ACh on the electric activity of rostral ventrolateral medullary neurons of rat in vitro

Chu Xiangping

Department of Physiology, Shanghai Medical University. Shanghai 200032, China


Extracellular single-unit discharges were obtained from l65 spontaneously active neurons within the region of the rostral ventrolateral medulla (RVLM) by glass microelectrode from 89 brain slices of the SpragUe-Dawley rats. The units could be divided into three types: regular (61.8%), irregular (24.2%) and silent (14%). Acetylcholine (ACh, 0.1, 0.3 #mu#mol/L) showed four kinds of effects on spontaneous discharges of RVLM neurons: excitatory, inhibitory, biphasic and non-responsive, counting respectively 41.8%, 20%, 3% and 35.2% of the neurons tested. The excitatory effect of ACh was dose-dependent. The effects, either excitatory or inhibitory,of ACh (n=49) were mostly blocked by atropine (0.3 #mu#mol/L, n=42). The excitatory effect of ACh (n=14) could be blocked mainly by selective antagonist of M_(1) receptor,pirenzepine (PZ, 30 nmol/L, n=9), but not by selective antagonist of M_(2) receptor,methoctramine (MT) and AFDX-116.

Key words: Acetylcholine;Brain slices of medulla;Rostral ventrolateral medulla;Spontaneous singleunit discharge;Subtype of muscarinic receptor

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储祥平 李鹏 徐宁善. 乙酰胆碱对离体大鼠延髓头端腹外侧区神经元自发放电的影响[J]. 生理学报 1997; 49 (6): .

Chu Xiangping. Effects of ACh on the electric activity of rostral ventrolateral medullary neurons of rat in vitro. Acta Physiol Sin 1997; 49 (6): (in Chinese with English abstract).