马兰1,2, 康奥琦1, 刘巍1, 聂晓军1, 田彦明1, 袁芳1,2,*
1河北医科大学生理学教研室，石家庄 050017；2河北省神经生理学重点实验室，石家庄 050017
大量研究表明孤束核神经元具有呼吸化学敏感特性，但是其分子机制仍有待揭示。本研究旨在观察电压门控钾离子通道Kv12在小鼠孤束核的表达。通过免疫荧光染色实验检测不同亚型Kv12 (Kv12.1，Kv12.2，Kv12.3)在孤束核的分布和细胞定位，用Western blot和定量PCR检测其不同亚型蛋白和mRNA在孤束核的表达差异。结果表明Kv12三种亚型在孤束核神经元都有表达，并且与Phox2b (paired-like homeobox 2b gene)存在共表达。三种亚型中Kv12.1 mRNA表达量最大，Kv12.3表达量最低。本研究结果表明小鼠孤束核Phox2b神经元表达Kv12，为孤束核神经元的pH敏感性提供了相关证据。
The Kv12 voltage-gated K+ channels are expressed in the Phox2b-expressing neurons in the nucleus tractus solitarii in mice
MA Lan1,2, KANG Ao-Qi1, LIU Wei1, NIE Xiao-Jun1, TIAN Yan-Ming1, YUAN Fang1,2,*
1Department of Physiology, Hebei Medical University, Shijiazhuang 050017, China；2Hebei Key Laboratory of Neurophysiology, Shijiazhuang 050017, China
Accumulating evidence demonstrates that the nucleus tractus solitarii (NTS) neurons serve as central respiratory chemoreceptors, but the underlying molecular mechanisms remain undefined. The present study investigated the expression of acid-sensitive ether-à-go-go-gene-like (Elk, Kv12) channels in the NTS of mice. Immunofluorescence staining was used to observe the distribution and cellular localization of the Kv12 channels in NTS neurons. Western blot and quantitative real-time PCR (qPCR) were used to evaluate protein and mRNA expression levels of Kv12 channels. The results showed that all of the three members (Kv12.1, Kv12.2, Kv12.3) of the Kv12 channel family were expressed in NTS neurons, and their expressions were co-localized with paired-like homeobox 2b gene (Phox2b) expression. The expression of Kv12.1 mRNA was the largest, whereas the expression of Kv12.3 was the least in the NTS. The results suggest Kv12 channels are expressed in Phox2b-expressing neurons in the NTS of mice, which provides molecular evidence for pH sensitivity in Phox2b-expressing NTS neurons.
通讯作者：袁芳 E-mail: email@example.com
马兰, 康奥琦, 刘巍, 聂晓军, 田彦明, 袁芳. 小鼠孤束核Phox2b神经元表达电压门控钾离子通道Kv12[J]. 生理学报 2021; 73 (2): 217-222.
MA Lan, KANG Ao-Qi, LIU Wei, NIE Xiao-Jun, TIAN Yan-Ming, YUAN Fang. The Kv12 voltage-gated K+ channels are expressed in the Phox2b-expressing neurons in the nucleus tractus solitarii in mice. Acta Physiol Sin 2021; 73 (2): 217-222