GluN2B-BDNF pathway in the cerebrospinal fluid-contacting nucleus mediates nerve injury-induced neuropathic pain in rats
WANG Yi-Wen1, ZHANG Yao-Yao1, WU Zheng-Yuan1, CHENG Er-Deng2, CHEN Yan-Qi1, SHEN Wen1, ZHANG Li-Cai1, ZHANG Su-Ming1,3,*
1Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical University, Xuzhou 221004, China；2Department of Anesthesiology, Shanghai Minhang Central Hospital, Shanghai 201100, China；3Department of Intensive Care Unit, The Affiliated Hospital of Xuzhou Medical University, Xuzhou 221009, China
The present study was aimed to investigate the role of GluN2B-BDNF pathway in the cerebrospinal fluid-contacting nucleus (CSF-CN) in neuropathic pain. Intra-lateral ventricle injection of cholera toxin subunit B conjugated with horseradish peroxidase (CB-HRP) was used to label the CSF-CN. Double-labeled immunofluorescent staining and Western blot were used to observe the expression of GluN2B and BDNF in the CSF-CN. Chronic constriction injury of sciatic nerve (CCI) rat model was used to duplicate the neuropathic pain. Pain behavior was scored to determine the analgesic effects of GluN2B antagonist Ro 25-6981 and BDNF neutralizing antibody on CCI rats. GluN2B and BDNF were expressed in the CSF-CN and their expression was up-regulated in CCI rats. Intra-lateral ventricle injection of GluN2B antagonist Ro 25-6981 or BDNF neutralizing antibody notably alleviated thermal hyperalgesia and mechanical allodynia in CCI rats. Moreover, the increased expression of BDNF protein in CCI rats was reversed by intra-lateral ventricle injection of Ro 25-6981. These results suggest that GluN2B and BDNF are expressed in the CSF-CN and alteration of GluN2B-BDNF pathway in the CSF-CN is involved in the modulation of the peripheral neuropathic pain.
Received: 2020-06-14 Accepted: 2020-09-21
Corresponding author: ZHANG Su-Ming E-mail: firstname.lastname@example.org,email@example.com
Citing This Article：
WANG Yi-Wen, ZHANG Yao-Yao, WU Zheng-Yuan, CHENG Er-Deng, CHEN Yan-Qi, SHEN Wen, ZHANG Li-Cai, ZHANG Su-Ming. GluN2B-BDNF pathway in the cerebrospinal fluid-contacting nucleus mediates nerve injury-induced neuropathic pain in rats. Acta Physiol Sin 2021; 73 (2): 223-232